Pedro Machado

Research Assistant

In 2008 I finished a degree in Anatomical Pathology in Escola Superior de Tecnologia da Saúde do Porto. During my degree, I was also an undergraduate trainee at the Advanced Tissue Analysis Facility, Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto.

During my traineeship at the IBMC I learned Transmission Electron Microscopy (TEM) techniques, and did Immuno-EM on mice samples.

Since I joined the CCR Lab I have been optimizing Electron Microscopy techniques for Drosophila melanogaster samples in order to perform ultrastructural analysis of centrioles, basal bodies, and axonemes.

If you enjoy and are familiar with ultrastructrure data, then you know that centrioles look awesome when analyzed by TEM, so being able to study such "eye candy" organelles gives me a lot of work motivation.

 

Publications

Carvalho-Santos Z, Machado P, Alvarez-Martins I, Gouveia SM, Jana SC, Duarte P, Amado T, Branco P, Freitas MC, Silva ST, Antony C, Bandeiras TM, Bettencourt-Dias M. (2012) BLD10/CEP135 is a microtubule-associated protein that controls the formation of the flagellum central microtubule pair. Dev Cell. 23(2):412-24. more

Jana SC, Machado P, Bettencourt-Dias M. (2012) A structural road map to unveil basal body composition and assembly. EMBO J. 31(3):519-21. more

Carvalho-Santos Z, Machado P, Branco P, Tavares-Cadete F, Rodrigues-Martins A, Pereira-Leal JB, Bettencourt-Dias M. (2012). Stepwise evolution of the centriole-assembly pathway. J Cell Sci. 123:1414-26. more

Martins AR, Machado P, Callaini G, Bettencourt-Dias M. (2010). Microscopy methods for the study of centriole biogenesis and function in Drosophila. Methods Cell Biol. more